Which of the following is not a desirable feature of a cloning vector?

Question

Question

Answer 1

Concept:

A cloning vector is a DNA molecule that acts as a vehicle to carry the gene of interest.
They carry the gene of interest into the host cell during the process of cloning.
The most common cloning vectors are plasmids, bacteriophages, cosmids, etc.

Explanation:

There are certain characteristics of a desirable vector used in gene cloning experiments.
Vector molecules should have a single recognition site.
This will help in easy ligation of the gene of interest at one specific location.
Multiple recognition sites will cut vector molecules at more than one position, leading to undesirable ligation of genes at multiple positions.
The vector should have the origin of replication so that it can replicate inside the host cell independently.
Should have selectable markers to identify the recombinants and non-recombinants.
Should have a single restriction enzyme site to cut the vector molecule at a specific location.

So, the correct answer is option 1.