In which of the following methods, recombinant DNA is entered directly into the nucleus of the animal cells?

This question was previously asked in
RPF SI (2018) Official Paper (Held On : 16 Jan 2019 Shift 3)
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  1. Inner Inactive 
  2. Micro Injection
  3. Bio Modification
  4. Gene Spike

Answer (Detailed Solution Below)

Option 2 : Micro Injection
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The correct answer is Micro Injection

Key Points

  • Micro Injection is the method where recombinant DNA is directly entered into the nucleus of animal cells.
  • This technique involves using a fine needle to inject DNA directly into the nucleus of the cell, ensuring precise delivery.
  • Micro Injection is commonly used in genetic engineering and reproductive biology to introduce new genetic material into cells.
  • This method is highly effective but requires specialized equipment and skills to perform correctly.

Additional Information

  • Recombinant DNA technology involves combining DNA from different sources to create a new genetic combination.
  • This technology has applications in medicine, agriculture, and research, including the production of insulin, genetically modified crops, and gene therapy.
  • Other methods of introducing recombinant DNA into cells include electroporation, liposome-mediated transfer, and viral vectors, but these methods do not necessarily deliver the DNA directly into the nucleus.
  • Micro Injection is particularly useful when high precision is required, such as in the creation of transgenic animals.
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